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cpa inhibitor  (MedChemExpress)


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    MedChemExpress cpa inhibitor
    Cpa Inhibitor, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/cpa inhibitor/product/MedChemExpress
    Average 94 stars, based on 3 article reviews
    cpa inhibitor - by Bioz Stars, 2026-02
    94/100 stars

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    Fig. 2. Fura-2 AM calcium imaging in mouse primary cultured urothelial cells (UCs). A: The differences in intracellular Ca2+ concentration after the application of DMSO (control); 10, 50, and 100 μM of PEA; 0.5 μM of <t>cyclopiazonic</t> acid <t>(CPA);</t> 5 μM of CID16020046 (CID); and extracellular Ca2+-free conditions in the mouse primary cultured UCs. B: Repre sentative traces of the Fura-2 ratio under each condition. Red arrows indi cate the application of each drug in B. The data are presented as means ± SE. The numbers of analyzed cells are 114, 151, 110, 121, 98, 72, 94, and 105 from the left column in A. Statistical analyses were done using Student's t-test. The level of statistical significance was set at P < 0.05. n.s., not significant, **P < 0.01.
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    Fig. 2. Fura-2 AM calcium imaging in mouse primary cultured urothelial cells (UCs). A: The differences in intracellular Ca2+ concentration after the application of DMSO (control); 10, 50, and 100 μM of PEA; 0.5 μM of <t>cyclopiazonic</t> acid <t>(CPA);</t> 5 μM of CID16020046 (CID); and extracellular Ca2+-free conditions in the mouse primary cultured UCs. B: Repre sentative traces of the Fura-2 ratio under each condition. Red arrows indi cate the application of each drug in B. The data are presented as means ± SE. The numbers of analyzed cells are 114, 151, 110, 121, 98, 72, 94, and 105 from the left column in A. Statistical analyses were done using Student's t-test. The level of statistical significance was set at P < 0.05. n.s., not significant, **P < 0.01.
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    Fig. 2. Fura-2 AM calcium imaging in mouse primary cultured urothelial cells (UCs). A: The differences in intracellular Ca2+ concentration after the application of DMSO (control); 10, 50, and 100 μM of PEA; 0.5 μM of cyclopiazonic acid (CPA); 5 μM of CID16020046 (CID); and extracellular Ca2+-free conditions in the mouse primary cultured UCs. B: Repre sentative traces of the Fura-2 ratio under each condition. Red arrows indi cate the application of each drug in B. The data are presented as means ± SE. The numbers of analyzed cells are 114, 151, 110, 121, 98, 72, 94, and 105 from the left column in A. Statistical analyses were done using Student's t-test. The level of statistical significance was set at P < 0.05. n.s., not significant, **P < 0.01.

    Journal: Life sciences

    Article Title: G protein-coupled receptor 55 activated by palmitoylethanolamide is associated with the development of nocturia associated with circadian rhythm disorders.

    doi: 10.1016/j.lfs.2023.122072

    Figure Lengend Snippet: Fig. 2. Fura-2 AM calcium imaging in mouse primary cultured urothelial cells (UCs). A: The differences in intracellular Ca2+ concentration after the application of DMSO (control); 10, 50, and 100 μM of PEA; 0.5 μM of cyclopiazonic acid (CPA); 5 μM of CID16020046 (CID); and extracellular Ca2+-free conditions in the mouse primary cultured UCs. B: Repre sentative traces of the Fura-2 ratio under each condition. Red arrows indi cate the application of each drug in B. The data are presented as means ± SE. The numbers of analyzed cells are 114, 151, 110, 121, 98, 72, 94, and 105 from the left column in A. Statistical analyses were done using Student's t-test. The level of statistical significance was set at P < 0.05. n.s., not significant, **P < 0.01.

    Article Snippet: A 0.5 μM of a specific inhibitor of ER Ca2+ ATPase (SERCA), cyclopiazonic acid (CPA) (Santa Cruz), and 5 μM of a selective GPR55 antagonist [30], CID16020046 (CID: Tocris Bioscience, Bristol, UK) were applied before T. Ihara et al. Life Sciences 332 (2023) 122072 5 min of PEA application.

    Techniques: Imaging, Cell Culture, Concentration Assay, Control

    Fig. 3. Measurement of isometric contraction or relaxation in an ex vivo mouse bladder and released ATP in primary cultured urothelial cells (UCs). A, The bladder strip contractile response for sequentially administered PEA from 0 to 100 μM into the organ bath system. B, Relaxation response of carbachol-induced contractions after sequentially administering CID16020046 from 0 to 1 mM into the organ bath system. C, The spontaneously released ATP from UCs after 5 min and 10 min of administering DMSO (control), 100 μM of PEA alone, and 100 μM of PEA and 0.5 μM of cyclopiazonic acid (CPA). D, The stretch released ATP from UCs after DMSO (control), PEA, and PEA and CPA applications. n = 8 in C and D. The data are presented as means ± SE. Statistical analyses were performed using a one-way ANOVA with the Bonferroni test. The level of statistical significance was set at P < 0.05. n.s., not significant. *P < 0.05. **P < 0.01.

    Journal: Life sciences

    Article Title: G protein-coupled receptor 55 activated by palmitoylethanolamide is associated with the development of nocturia associated with circadian rhythm disorders.

    doi: 10.1016/j.lfs.2023.122072

    Figure Lengend Snippet: Fig. 3. Measurement of isometric contraction or relaxation in an ex vivo mouse bladder and released ATP in primary cultured urothelial cells (UCs). A, The bladder strip contractile response for sequentially administered PEA from 0 to 100 μM into the organ bath system. B, Relaxation response of carbachol-induced contractions after sequentially administering CID16020046 from 0 to 1 mM into the organ bath system. C, The spontaneously released ATP from UCs after 5 min and 10 min of administering DMSO (control), 100 μM of PEA alone, and 100 μM of PEA and 0.5 μM of cyclopiazonic acid (CPA). D, The stretch released ATP from UCs after DMSO (control), PEA, and PEA and CPA applications. n = 8 in C and D. The data are presented as means ± SE. Statistical analyses were performed using a one-way ANOVA with the Bonferroni test. The level of statistical significance was set at P < 0.05. n.s., not significant. *P < 0.05. **P < 0.01.

    Article Snippet: A 0.5 μM of a specific inhibitor of ER Ca2+ ATPase (SERCA), cyclopiazonic acid (CPA) (Santa Cruz), and 5 μM of a selective GPR55 antagonist [30], CID16020046 (CID: Tocris Bioscience, Bristol, UK) were applied before T. Ihara et al. Life Sciences 332 (2023) 122072 5 min of PEA application.

    Techniques: Ex Vivo, Cell Culture, Stripping Membranes, Control